Journal: Frontiers in Immunology

Article Title: Mitochondrial dysfunction and immune microenvironment in gestational diabetes mellitus: insights from bioinformatics analysis and experimental validation

doi: 10.3389/fimmu.2026.1771616

Figure Lengend Snippet: Single-cell resolution reveals the expression of hub mitochondrial-related genes (Mito-RGs) and the immune landscape in gestational diabetes mellitus (GDM). (A) Uniform manifold approximation and projection (UMAP) plot presenting distinct cell clusters identified from single-cell RNA sequencing (scRNA- seq ) data. (B) UMAP plot displaying the distribution of cells across different individual samples, including GDM and control groups. (C) UMAP plot comparing cell distributions between GDM and control groups. (D) Bubble plot highlighting the top five marker genes for each cell cluster, aiding in the identification of major cell types. (E) UMAP plot with annotated cell types, including tissue stem cells, epithelial cells, macrophages, monocytes, neutrophils, natural killer (NK) cells, B cells, endothelial cells, myelocytes, and common myeloid progenitors (CMPs). (F) Bar plot illustrating the proportions of different identified cell types in each sample. (G) Bar plot comparing the proportions of major cell types between the GDM and control groups. (H) Feature plots depicting the expression patterns of hub Mito-RG (DHRS2, STX17, and TIMM44) in specific cell clusters. (I) Feature plot showing the distribution of Mito-RGs scores across various cell subpopulations, reflecting their enrichment in particular immune and stromal cell types.

Article Snippet: Overnight incubation of tissue sections at 4 °C was performed with primary antibodies: DHRS2 (Proteintech, 15735-1-AP), STX17 (Affinity Biosciences, DF12483), and TIMM44 (Affinity Biosciences, DF12332).

Techniques: Single Cell, Expressing, RNA Sequencing, Control, Marker

Journal: Frontiers in Immunology

Article Title: Mitochondrial dysfunction and immune microenvironment in gestational diabetes mellitus: insights from bioinformatics analysis and experimental validation

doi: 10.3389/fimmu.2026.1771616

Figure Lengend Snippet: Cell-cell communication analysis and experimental validation of hub mitochondrial-related genes (Mito-RGs) expression. (A) Heatmap of gene set variation analysis (GSVA) enrichment across different cell subtypes. (B) Cell-cell communication network diagram illustrating interactions among various cell subtypes. (C-E) Ligand-receptor pair analysis of key signaling pathways: (C) TGFB1-TGFBR1/TGFBR2, (D) FN1-ITGA5/ITGB1, and (E) LAMA5-CD44. (F) Comparison of body weight changes between gestational diabetes mellitus (GDM) and control mice. (G) Comparison of blood glucose levels between GDM and control mice at different time points during the oral glucose tolerance test (OGTT). (H-J) Immunohistochemistry (IHC) staining and scoring of DHRS2 (H) , STX17 (I) , and TIMM44 (J) in placental tissues from GDM and control mice.

Article Snippet: Overnight incubation of tissue sections at 4 °C was performed with primary antibodies: DHRS2 (Proteintech, 15735-1-AP), STX17 (Affinity Biosciences, DF12483), and TIMM44 (Affinity Biosciences, DF12332).

Techniques: Biomarker Discovery, Expressing, Protein-Protein interactions, Comparison, Control, Immunohistochemistry

Journal: Translational Pediatrics

Article Title: Untargeted lipidomics of bronchopulmonary dysplasia induced by hyperoxia exposure in rats

doi: 10.21037/tp-23-546

Figure Lengend Snippet: Alveoli in rats is impaired with hyperoxia. (A) Representative images of H&E-stained tissue slices describing alveolation 14 days after birth in rats. Arrowheads represent rat alveolar area. (B) Summary data of quantitative histomorphometric analyses: RAC. (C) Representative images of histochemical stained tissue slices describing the expression of the pulmonary SFTPD 14 days after birth in rats. The arrowheads represent cells that express SFTPD protein. (D) Quantitative analysis of immunohistochemistry results. t -test; ***, P<0.001. H&E, hematoxylin-eosin; SFTPD, surfactant-associated protein D; RAC, radial alveolar count.

Article Snippet: Use phosphate-buffered saline (PBS) containing 1.5% rabbit serum to block for 30 minutes at room temperature, then add goat anti-rabbit antibody the pulmonary surfactant-associated protein D (SFTPD; Proteintech, Wuhan, China; 1:100 dilution) to the slide and incubate overnight at 4 ℃.

Techniques: Staining, Expressing, Immunohistochemistry